Team

    • Dr Franck DEBARBIEUX (MCU AMU)
    • Dr Ivo VANZETTA (CR, CNRS)
    • Pr Geneviève ROUGON (DRCE émérite CNRS)

Location

  • Labs : basement and 1st floor (Level 0 and +1)
  • Office : Tertiary Deptt (Level +1)
  • Contact us

Purposes and Missions

The Optical Department aims to provide a unique characterization of neuroinflammatory processes at play in mice models of neuropathologies (glioblastoma brain tumors, multiple sclerosis, CNS trauma).

This is achieved by the integration, development and use of the most up-to-date optical technologies to image the dynamic cellular composition of the pathological central nervous system in control and treated conditions. We also aim at tuning optical methodologies for their use in combination with implantable stimulating/recording electronic devices for Human Machine Interface

Media

CT imaging of a mouse bearing a GFP-expressing GBM implanted in cortex

Fluorescent macroscope imaging of a mouse bearing a GFP-expressing GBM implanted in cortex

2P imaging of a mouse bearing a GFP-expressing GBM implanted in cortex

Biphoton microscopy

Fluorescence microscopy with infrared LASER scanning in straight configuration above a micrometric motorized deck, capable of receiving a stereotaxy device for rodents under the objective. A thermostated surrounding wall at 34°C surrounds the motorized deck. The femtosecond pulsed infrared LASER comes with several watts and is compatible with the 700-1020nm range to allow an optimal excitement of a broad selection of fluorophores. The adjustable intensity (1% precision) between 0 and 100 % allows the imaging on >700µm depth in vivo in the brain. The objectives for IR until 1020nm have a digital opening superior to 0.8 and magnify sufficiently to obtain a submicrometric definition of the image on a field >400µm. The detection system of the very sensitive fluorescence allows to acquire simultaneously several colors to follow the dynamics of the interactions between several populations of cells on alive animals.

Références

1. Ricard, C., Stanchi, F., Rougon, G., Debarbieux, F. An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy. J. Vis. Exp. (), e51108, doi:10.3791/51108 (2014). Jove Video

2. Ricard C, Stanchi F, Weber P, Rougon G, Debarbieux F. An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy J Vis Exp. 2014 (in press)

3. Ricard C, Debarbieux FC. Six-color intravital two-photon imaging of brain tumors and their dynamic microenvironment. Front Cell Neurosci. 2014 Feb 24;8:57. doi: 10.3389/fncel.2014.00057.

4. Fenrich KK, Weber P, Rougon G, Debarbieux F.Implanting glass spinal cord windows in adult mice with experimental autoimmune encephalomyelitis. J Vis Exp. 2013 Dec 21;(82):e50826. doi: 10.3791/50826
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.5. Ricard C, Stanchi F, Rodriguez T, Amoureux MC, Rougon G, Debarbieux F. Dynamic Quantitative Intravital Imaging of Glioblastoma Progression Reveals a Lack of Correlation between Tumor Growth and Blood Vessel Density. PLoS One. 2013 Sep 12;8(9):e72655

6 . Fenrich KK, Weber P, Rougon G, Debarbieux F. (2013) Long and short term intravital imaging reveals differential spatiotemporal recruitment and function of myeloid cells after spinal cord injury J Physiol.; 2013 Sep 9.

7. Fenrich KK, Weber P, Hocine M, Zalc M, Rougon G, Debarbieux F. (2012) Long‐term in vivo imaging of normal and pathological mouse spinal cord with subcellular resolution using implanted glass windows. J Physiol.; 590:3665-­‐75.